Abstract

Overview

The increased exposure to electromagnetic waves has fueled concerns and debates due to the unclear biological impacts these radiation levels pose, particularly in terms of environmental and human health. This study provides an analytical approach to assess the effects of 100 MHz radiofrequency electromagnetic field (RF-EMF) on zebrafish embryos, leveraging a multi-disciplinary protocol.

Research Methods

• Use of zebrafish as a model due to its genomic similarities with humans.
• Development of a precise exposure and measurement protocol to clearly distinguish between biological and thermal effects of RF-EMF.

Findings

Observations from 24 to 72 hours post-fertilization showed significant effects on embryonic development:

• Reduced growth and increased transcription of oxidative stress genes at 48 hours.
• Triggering of apoptotic and autophagic processes.
• Alterations in cholesterol metabolism.
• Initiation of detoxification mechanisms allowing partial recovery by 72 hours.

Conclusion

The data conclusively demonstrate the non-thermal in vivo effects of 100 MHz EMF on zebrafish embryonic development. These findings underscore the biological impact of RF-EMF exposure and pave the way for future studies to explore the dose-response relationships of electromagnetic fields.