ZIP9-centered pathway compensates for radiofrequency radiation-mediated reduction in resistance to H2O2 damage in Sertoli cells of rats

Authors: Yu G, Zhu Y, Song C, Chen L, Tang Z, Wu T

Year: 2023 Mar 6

Category: Ecotoxicology

Journal: Ecotoxicol Environ Saf

Institution: Ecotoxicol Environ Saf

DOI: 10.1016/j.ecoenv.2023.114733

URL: https://pubmed.ncbi.nlm.nih.gov/36889209/

Abstract

Overview

The study delves into the impacts of 2605 MHz radiofrequency electromagnetic radiation (RF-EMR) on the testes, specifically considering its effects on Sertoli cells in rats. This research builds on prior findings that long-term RF-EMR exposure could harm spermatogenesis and induce reproductive toxicity.

Findings

  • Short-term Exposure: The research established a scrotal exposure model for rats, showing that short-term RF-EMR exposure did not significantly affect sperm quality or spermatogenesis. However, it did increase the levels of testicular testosterone and zinc transporter 9 (ZIP9), which play protective roles in Sertoli cells.
  • Protective Role of Testosterone: Testosterone (T) reversed the increases in apoptosis rates and malondialdehyde (MDA) levels in Sertoli cells exposed to H2O2, by enhancing ZIP9 levels. Additionally, the suppression of ZIP9 expression notably reduced these protective effects by testosterone.
  • Molecular Mechanisms: Enhancements in molecular pathways involving phosphorylated inositol-requiring enzyme 1 (P-IRE1), eukaryotic initiation factor 2a (P-eIF2a), among others, were observed due to testosterone’s action, which were reversed on ZIP9 inhibition.
  • Long-term Changes: With prolonged exposure, a gradual decrease in ZIP9 and increase in MDA was observed in the testes, indicating deteriorating conditions over time.

Conclusion

The study contributes to understanding the complex mechanisms by which short-term exposure to RF-EMR affects Sertoli cells and outlines the key role of the ZIP9-centered androgen pathway in mitigating these effects. This is crucial for uncovering the time-dependent reproductive toxicity associated with RF-EMR.

radiofrequency electromagnetic radiation Sertoli cells testosterone ZIP9 reproductive toxicity RF-EMR spermatogenesis
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