1950 MHz RF EMR Inhibits Testosterone Secretion of Mouse Leydig Cells

Abstract

Overview

This study delves into the effects of 1950 MHz radio-frequency (RF) electromagnetic radiation (EMR) on mouse Leydig (TM3) cells. The research particularly focuses on the bioeffects caused by RF EMR emitted from communication devices.

Methodology

• Duration & Exposure: TM3 cells underwent 24 hours of continuous irradiation at a specific absorption rate (SAR) of 3 W/kg.
• Evaluation Techniques: Post-radiation effects were assessed using various methods including the Cell Counting Kit-8 (CCK-8) for cell proliferation, flow cytometry for cell cycle distribution and apoptosis, ROS measurements, and enzyme-linked immunosorbent assay (ELISA) for Testosterone levels.
• Gene Expression Analysis: Real-time PCR was deployed to examine mRNA levels of steroidogenic acute regulatory protein (StAR) and P450scc in TM3 cells.

Findings

The exposure to 1950 MHz RF radiation at 3 W/kg resulted in:

• Noticeable decrease in cell proliferation.
• Reduction in testosterone levels and P450scc mRNA expression.
• Unchanged levels in cell apoptosis and StAR mRNA expression.

Conclusion

The data suggests that 24-hour exposure to 1950 MHz RF EMR can significantly impact cell proliferation and testosterone secretion in mouse Leydig cells. Highlighting potential health risks, further investigation into the reproductive effects of RF radiation is crucial.