Abstract

Overview

To investigate the impact of high-frequency (HF-EMF) and low-frequency (LF-EMF) electromagnetic fields on neural stem cells (NSCs) derived from the hippocampus of SD rats, examining their effects on proliferation and differentiation.

Methodology

• NSCs cultured in suspension and adherent differentiation media.
• Exposure protocols: LF-EMF group (5 mT, 50 Hz, 30 min daily); HF-EMF group (maximum magnetic induction 2.5 T, 40% MO, 50 Hz, 10 min daily); Control group with no electromagnetic field.

Findings

• Assessment via CCK-8 assay and cell counting showed enhanced cell viability and proliferation in LF-EMF and HF-EMF exposed groups.
• Immunofluorescence and qRT-PCR analysis indicated higher Tuj-1 expression in LF-EMF group, suggesting heightened differentiation into neurons.
• GFAP expression did not significantly differ among the groups, indicative of unaffected astrocyte differentiation.

Conclusion

Both LF-EMF and HF-EMF facilitate the proliferation of NSCs in vitro. Specifically, LF-EMF accelerates differentiation into neurons, highlighting the potential of EMF in therapeutic applications for brain disorders but also pointing to the need for careful consideration of EMF exposure levels.