Comparison of effects of high- and low-frequency electromagnetic fields on proliferation and differentiation of neural stem cells

Authors: Wenfang Bai, Meihui Li, Weicheng Xu, Mingsheng Zhang

Year: 2021 Jan 10

Category: Neuroscience

Journal: Neurosci Lett

Institution: Neurosci Lett

DOI: 10.1016/j.neulet.2020.135463

URL: https://pubmed.ncbi.nlm.nih.gov/33129846/

Abstract

Overview

This study examines the impact of both high-frequency (HF-EMF) and low-frequency electromagnetic fields (LF-EMF) on the proliferation and differentiation of neural stem cells (NSCs) extracted from the hippocampus of SD rats.

Methodology

  • NSCs were cultured in both suspension and adherent differentiation media.
  • Exposure conditions included LF-EMF (5 mT, 50 Hz, 30 min daily), HF-EMF (maximum magnetic induction 2.5 T, 40% MO, 50 Hz, 10 min daily), and a control group with no electromagnetic field.
  • Cell viability and quantity were assessed using CCK-8 assay and cell counting plate after 3 days.

Findings

  • Immunofluorescence staining and qRT-PCR were utilized to analyze cell markers and gene expression.
  • Both LF-EMF and HF-EMF promoted NSC proliferation; LF-EMF also enhanced differentiation into neurons.
  • The LF-EMF group showed significantly higher proliferation and neuron differentiation indices compared to the HF-EMF and control groups (P < 0.05).

Conclusion

The study underscores the potential risks associated with electromagnetic field exposure, showing significant biological effects on neural stem cells. Such findings are crucial for understanding EMF impacts on biological systems and reinforce the need for stringent regulations and protective measures against EMF exposures.

neural stem cells electromagnetic fields proliferation differentiation neuroscience EMF safety
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