Abstract

Study Overview

This study investigates the effects of a 50-Hz magnetic field (MF) on human amniotic epithelial (FL) cells, focusing on the activation pathway of sphingosine kinase 1 (SK1) and associated cellular processes.

Main Findings

• MF exposure results in increased intracellular calcium levels, dependent on L-type calcium channels.
• Calcium-dependent phosphorylation of extracellular regulated protein kinase (ERK), SK1, and protein kinase C α (PKCα) was observed.
• Using U0126, an ERK inhibitor, blocked MF-induced SK1 phosphorylation but did not affect PKCα phosphorylation.
• The inhibitor of PKCα, Gö6976, did not influence MF-induced SK1 activation.
• Conversely, inhibition of SK1 by SKI II halted both ERK and PKCα activation.

Conclusion

The study concludes that intracellular calcium mediates 50-Hz MF-induced SK1 activation, enhancing PKCα phosphorylation. Additionally, potential feedback mechanisms between SK1 and ERK activation in response to MF exposure were identified in FL cells, highlighting a complex interaction within cellular signaling pathways influenced by MF exposure.