Abstract

Overview

The rapid growth of wireless electronic devices has raised concerns about the harmful effects of leaked electromagnetic radiation (EMR) on human health. This study investigates the biological effects of radio frequency (RF) EMR on oligodendrocytes amidst unclear conclusions from numerous previous studies.

Methodology

• Oligodendroglial and three other types of brain cells were exposed to 2.4 GHz EMR for either 6 or 48 hours, at an average input power of 1 W.
• The exposure was in the form of continuous wave (CW-RF) or pulse-modulated wave (PW-RF) with a 50 Hz pulse frequency and 1/3 duty cycle.
• Techniques such as RNA sequencing, RT-qPCR, and Western blot were used to analyze gene expression.
• Multiple reaction monitoring (MRM) examined the expression levels of C/EBPβ-interacting proteins.

Findings

Pulse-Modulated RF EMR significantly increased the mRNA levels of C/EBPβ in oligodendroglia, indicating an observable impact on specific cell types and gene expressions under controlled RF exposure.

Conclusion

The 2.4 GHz frequency, prevalent in various modern electronic devices, influences gene expression and function in oligodendrocytes through pulse-modulated exposure. The findings highlight a potential underexplored area of electromagnetic influence on biological functions, suggesting a need to further explore environmental EMF impacts on health.