Label-Free Study of the Global Cell Behavior during Exposure to Environmental Radiofrequency Fields in the Presence or Absence of Pro-Apoptotic or Pro-Autophagic Treatments

Authors: Joushomme A, Garenne A, Dufossée M, Renom R, Ruigrok HJ, Chappe YL, Canovi A, Patrignoni L, Hurtier A, Poulletier de Gannes F, Lagroye I, Lévêque P, Lewis N, Priault M, Arnaud-Cormos D, Percherancier Y

Year: 2022 Jan 8

Category: Molecular Science

Journal: Int J Mol Sci

Institution: Int J Mol Sci

DOI: 10.3390/ijms23020658

URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8776001/

Abstract

Overview

The impact of environmental radiofrequency signals on cellular behavior, particularly in contexts of cellular stress like apoptosis or autophagy, remains a disputed topic within molecular science. This study seeks to elucidate any potential effects using innovative approaches.

Methodology

Two human cell lines and two rat cortex cell cultures were exposed to a range of radiofrequency (RF) environments, such as GSM, UMTS, LTE, Wi-Fi, and continuous wave signals, with various modulation patterns. These experiments employed sophisticated, label-free techniques including cellular impedancemetry and Digital Holographic Microscopy.

Experimental Conditions

S.A.R. (Specific Absorption Rates): Ranging from 1.5 to 24 W/kg.
Continuous exposure durations spanned three to five consecutive days.
Cellular responses were measured at a constant temperature.

Findings

No significant changes were observed in the global behavior of cells under any conditions, irrespective of SAR levels exceeding current guidelines, as long as exposure temperature remained regulated.

Conclusion

This comprehensive analysis suggests that while exposure to various types of RF signals does not seem to influence cellular behavior directly, the electromagnetic field safety continues to necessitate cautious evaluation, especially under variable environmental conditions and in combination with other cellular stressors.

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