A microfabricated lab-on-chip with three-dimensional electrodes for microscopic observation of bioelectromagnetic effects of cells

Abstract

Overview

Exploring the effects of electromagnetic (EM) signals, which are prevalent in electronic instruments and biomedical systems, on biological structures, this research develops a novel microfabricated lab-on-chip. This device integrates three-dimensional interdigital electrodes designed to facilitate microscopic observations of cell behaviors under EM influence.

Method and Simulation

The lab-on-chip was conceived to deliver low-frequency EM stimulation suitable for cell observation through a microscope. The device employs an input signal of 10Vpp at 10 kHz, achieving an electric field strength of 4.45 Vrms/m in the culture medium. This setup allows for estimations of field strength across frequencies up to 3 MHz via simulations.

Fabrication and Testing

• A full-wafer microfabrication technique was utilized to create a prototype.
• Impedance measurements conducted between 20 Hz and 30 MHz confirm the prototype's operational range.
• The human cell line HEK293T was cultured within the device over a 24-hour period to test biocompatibility and the effects of EM exposure.

Findings

Observational results from the microscopy indicate that cell growth is significantly influenced by the 10 kHz EM signal, suggesting potential implications for understanding how human bodies might interact with electromagnetic fields in various environments.

Conclusion

The fabricated lab-on-chip proves effective for long-term microscopic studies of the EM effects on biological structures, highlighting important discoveries about cell behavior in response to electromagnetic fields.