Effects of 220 MHz Pulsed Modulated Radiofrequency Field on the Sperm Quality in Rats

Authors: Guo L, Lin JJ, Xue YZ, An GZ, Zhang JP, Zhang KY, He W, Wang H, Li W, Ding GR

Year: 2019 Apr 10

Category: Environmental Health

Journal: Int J Environ Res Public Health

DOI: 10.3390/ijerph16071286

URL: https://www.ncbi.nlm.nih.gov/pubmed/30977029

Abstract

Overview

This study focuses on the impact of a high-intensity 220 MHz pulsed modulated RF field, encountered in certain occupational settings, on the reproductive health of male adult rats. Researchers sought to determine the implications of prolonged exposure to these conditions.

Findings

Exposure parameters: One month at a power density of 50 W/m².
Primary evaluations included sperm cell number, abnormalities, survival rate, and testicular morphology.
Key techniques used: Hematoxylin-eosin staining, ELISA for Sertoli and Leydig cell factors, immunofluorescence staining for cleaved caspase 3, and Western blotting for apoptosis-related proteins.

Conclusion

Exposure to 220 MHz pulsed modulated RF resulted in decreased sperm quality. Significant changes included reduced secreting factors in Leydig cells, altered testis morphology, and an increased rate of apoptosis in testis cells. The findings emphasize the potential health risks associated with occupational exposure to RF fields.

radiofrequency fields sperm quality rat study apoptosis testis morphology occupational health enzyme assays

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