Assessment of DNA Damage in Chick Embryo Brains Exposed to 2G and 3G Cell Phone Radiation using Alkaline Comet Assay Technique

Abstract

Overview

Cellular and mobile phones have quickly become ubiquitous, stirring concerns about the health implications of Radiofrequency Radiation (RFR) especially in relation to brain development in embryos.

Aim

The study focuses on the possible Deoxyribonucleic Acid (DNA) damage in the brains of chick embryos subjected to chronic exposure from 2G and 3G cell phone radiation.

Materials and Methods

• Fertilised hen eggs divided into: Group A (2G exposure), Group B (3G exposure), and Group C (control).
• Exposure duration was 72 minutes daily, with evaluations made from the 9th to the 12th day post exposure.
• DNA damage was assessed using the alkaline comet assay technique.

Findings

Statistical analysis showed a significant increase in DNA damage metrics such as comet length, tail length, percentage of DNA in the tail, and tail moment, with differences more pronounced under 3G exposure.

Conclusion

The findings suggest substantial DNA damage in chick embryo brains, highlighting potential risks of developmental exposure to cell phone radiation, especially under frequency conditions used by 3G networks.