Histopathological and epigenetic alterations in the spinal cord due to prenatal electromagnetic field exposure: An H3K27me3-related mechanism

Abstract

Overview

Neural system development is critical during embryogenesis, with genetic and environmental factors influencing the presence of neural tube defects and other central nervous system diseases. This study focuses on the prenatal exposure to 900-MHz electromagnetic fields and its potential health risks.

Methodology

Pregnant rats were subjected to 900-MHz EMF for one hour daily from embryonic day 13.5 until birth. Postnatally, on day 32, samples from the upper thoracic region of the spine were collected and analyzed.

Findings

• Structural changes in ependymal cells, enlargement of central canals, and degeneration of motor neurons were observed in EMF exposed groups.
• Histological staining methods were used, including Hematoxylin & Eosin for general morphology and Cresyl Violet for neuronal quantitation.
• The control group displayed normal structural appearances, whereas the EMF group showed low levels of H3K27me3 immunoactivity indicating potential disruptions in neurodevelopment and stem cell potential.

Conclusion

This research highlights the detrimental effects of prenatal exposure to electromagnetic fields on the spinal cord, implicating H3K27me3 as a key molecular player in these adverse changes.