Abstract

Overview

The increasing use of nonionizing radiofrequency electromagnetic fields (RF-EMFs) across various technologies calls for a deeper understanding of their biological effects. This study focuses on how RF-EMFs influence cellular epigenetic mechanisms, particularly DNA methylation, which regulates gene expression dynamically in response to external stimuli.

Methodology

The study involved exposing human keratinocytes to a 900 MHz RF-EMF for one hour at a low dose rate (mean specific absorption rate < 10 mW/kg) using a custom system that maintains biologically relevant conditions (37 °C, 5% CO2, 95% humidity). Immediate changes in DNA methylation patterns were assessed using whole genome bisulfite sequencing.

Findings

• Identification of immediate changes in DNA methylation patterns.
• Correlation of these changes with global gene expression to pinpoint differentially methylated and expressed genes.
• Highlighting six potential epigenetic biomarkers responsive to RF-EMF exposure.

Conclusion

The results suggest a significant epigenetic role in cellular responses to RF-EMFs, emphasizing the need for further studies on the potential health risks associated with RF-EMF exposure.