Low-frequency electromagnetic fields influence the expression of calcium metabolism related proteins in leukocytic cell lines

Authors: Wójcik-Piotrowicz K, Kaszuba-Zwoińska J, Piszczek P, Nowak B, Guzdek P, Gil K, Rokita E

Year: 2023

Category: Toxicology

Journal: Environ Toxicol Pharmacol

DOI: 10.1016/j.etap.2023.104320

URL: https://pubmed.ncbi.nlm.nih.gov/37984675/

Abstract

Overview

This study assesses the effects of low-frequency magnetic fields (LF-MFs) on the viability of leukocytic cell lines through changes in calcium metabolism proteins mediated by calcineurin (CaN) signaling pathways and reactive oxygen species (ROS).

Methodology

  • Cell Lines Used: Mono Mac 6 and U937.
  • Stress Stimuli: Exposure to various LF-MFs and puromycin (PMC).
  • Evaluation Techniques: Protein expression analyzed by Western Blot, ROS levels and cell viability assessed using cytochrome C reduction assay and flow cytometry respectively.

Findings

The interaction between applied MF and PMC resulted in MF-dependent changes in cell viability. These changes were directly associated with protein expression and ROS levels, indicating cell susceptibility to undergo cell death upon exposure to stress stimuli.

Conclusion

The study confirms that specific bioeffects related to LF-MFs are distinguishable in different leukocyte populations, supporting the hypothesis that LF-MF exposure can influence cell behavior through CaN-mediated pathways and ROS production.

electromagnetic fields calcium metabolism leukocytic cell lines protein expression ROS
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