Proteomic Analysis of the Effect of ELF-EMF With Different Intensities in SH-SY5Y Neuroblastoma Cell Line

Authors: Rezaie-Tavirani M, Hasanzadeh H, Seyyedi S, Zali H

Year: 2017 Spring

Category: Proteomics

Journal: J Lasers Med Sci

Institution: J Lasers Med Sci

DOI: 10.15171/jlms.2017.14

URL: https://www.ncbi.nlm.nih.gov/pubmed/28652900

Abstract

Abstract Summary

Overview

Human exposure to extremely low frequency electromagnetic fields (ELF-EMF), emitted by power lines and electronic devices, has been a subject of research for the last 30 years, raising concerns about potential biological effects.

Investigation Purpose

The study focuses on the impact of 50 Hz ELF-EMF at different intensities on SH-SY5Y human neuroblastoma cell line, examining changes in cell morphology and protein expression.

Methods Employed

  • Exposure of SH-SY5Y cells to 0.5 and 1 mT 50 Hz ELF-EMFs for 3 hours.
  • Utilization of proteomics techniques to evaluate the effect on protein expression.
  • Bioinformatic and statistical analysis using Progensis SameSpots software.

Findings

Results indicate that ELF-EMF exposure results in:

  • Alterations in cell morphology.
  • A dose-dependent decrease in cell proliferation rates.
  • Changes in cell protein expression, intensifying with increased field intensity.

Conclusion

Increased intensity of ELF-EMF is linked to more pronounced alterations in cell protein expression along with effects on cell morphology and proliferation, suggesting potential health risks associated with ELF-EMF exposure.

ELF-EMF proteomics neuroblastoma cell morphology protein expression electromagnetic fields bioinformatics
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