Abstract

Overview

Extracellular vesicles (EVs) are pivotal in cellular communication, carrying molecular cargos to influence recipient cell behavior. Their application in diagnostics and therapy for various diseases underscores their significance in medical research.

Hypothesis

This study explores the impact of electric fields (EFs) on the release and content of EVs. It utilizes a specialized bioreactor that mimics in-vivo conditions within programmable EF environments to support this investigation.

Methodology

• Development of a three-dimensional cell culture environment.
• Implementation of a three-step EV purification protocol for high-density EV production.
• Utilization of mass spectrometry-based proteomics and high-resolution nanoparticle flowcytometry for detailed analysis.

Findings

Electrical stimulation similar to that used in therapeutic deep brain stimulation influences EV release and cargo content in a frequency-dependent manner. This impact suggests a novel approach to manipulating EV production through electrical fields.

Conclusion

This research provides new insights into how electrical activity influences EV production and properties. It proposes potential mechanisms by which therapeutic electrical stimulations could treat brain disorders and offers novel methods for EV cargo generation in controlled settings.