Effects of extremely low-frequency magnetic fields on human MDA-MB-231 breast cancer cells: proteomic characterization

Authors: Lazzarini R, Eléxpuru-Zabaleta M, Piva F, Giulietti M, Fulgenzi G, Tartaglione MF, Zingaretti L, Tagliabracci A, Valentino M, Santarelli L, Bracci M

Year: 2023 Feb 16

Category: Cell Biology

Journal: Ecotoxicol Environ Saf

Institution: Ecotoxicol Environ Saf

DOI: 10.1016/j.ecoenv.2023.114650

URL: https://pubmed.ncbi.nlm.nih.gov/36805133

Abstract

Overview

Extremely low-frequency electromagnetic fields (ELF-MF) have been examined for potential effects on the cell viability and regulatory processes in various cell types, including breast cancer cells. Recent research suggests a significant interaction with cellular components, which may underline a potential health risk.

Findings

  • ELF-MF at 50 Hz and 1 mT for 4 hours alters cell viability and increases the live cell number in human breast cancer cells (MDA-MB-231).
  • Significant changes were observed in the cellular morphology, such as increased density and length of filopodia.
  • ELF-MF exposure led to increased mitochondrial ROS levels and altered mitochondrial morphology.
  • The proteomic profile was extensively modified by ELF-MF, affecting 328 proteins in MDA-MB-231 cells, with key impacts noted in cellular adhesion, migration, and invasion.
  • The upregulation of transcription factors associated with cellular reprogramming indicates profound biochemical and physiological alterations.

Conclusion

This study underscores the potential of ELF-MF to influence crucial biological properties of breast cancer cells, enhancing our understanding of their multifactorial nature and pointing towards ELF-MF as a factor that could aggravate the cellular characteristics associated with malignancy.

ELF-MF breast cancer proteomics cell viability oxidative stress cellular morphology signaling pathways
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