Static Magnetic Stimulation Induces Changes in the Oxidative Status and Cell Viability Parameters in a Primary Culture Model of Astrocytes

Authors: da Costa CC, Martins LAM, Koth AP, Ramos JMO, Guma FTCR, de Oliveira CM, Pedra NS, Fischer G, Helena ES, Gioda CR, Sanches PRS, Junior ASV, Soares MSP, Spanevello RM, Gamaro GD, de Souza ICC

Year: 2021 Jun 27

Category: Cell Biology, Biophysics

Journal: Cell Biochem Biophys

DOI: 10.1007/s12013-021-01015-7

URL: https://pubmed.ncbi.nlm.nih.gov/34176101/

Abstract

Abstract Summary

Overview

Astrocytes, vital for central nervous system function and brain plasticity, are influenced by static magnetic fields (SMF) during brain therapy. This study focuses on the cellular-level impacts of SMF.

Findings

  • Exposure: Primary culture of cortical astrocytes from neonatal Wistar rats was exposed to SMF at 305 mT for varying durations over 7 consecutive days.
  • Effects on Cell Viability and Oxidative Stress: Significant changes were observed in oxidative stress markers and cell viability. Antioxidant capacity decreased notably in groups stimulated for 5 and 40 minutes.
  • Lipid Peroxidation: Increased across all stimulated groups, indicating heightened oxidative stress.
  • Cell Cycle: Significant alterations after 40 minutes of exposure, with increased necrosis noted at 15, 30, and 40 minutes.
  • Mitochondrial Function: Overall reduction post-stimulation, although no drastic changes in the mitochondrial network were visually confirmed.

Conclusion

The results underscore significant concerns regarding the use of SMF in therapeutic settings, particularly highlighting compromised cellular integrity and function in stimulated astrocytes. This raises important safety considerations for SMF use in treating neurological conditions.

astrocytes static magnetic fields cell viability oxidative stress neurology
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