Static Magnetic Stimulation Induces Changes in the Oxidative Status and Cell Viability Parameters in a Primary Culture Model of Astrocytes

Authors: da Costa CC, Martins LAM, Koth AP, Ramos JMO, Guma FTCR, de Oliveira CM, Pedra NS, Fischer G, Helena ES, Gioda CR, Sanches PRS, Junior ASV, Soares MSP, Spanevello RM, Gamaro GD, de Souza ICC

Year: 2021 Jun 27

Category: Cell Biology

Journal: Cell Biochem Biophys

DOI: 10.1007/s12013-021-01015-7

URL: https://pubmed.ncbi.nlm.nih.gov/34176101/

Abstract

Overview

Astrocytes are crucial for central nervous system functionality and brain plasticity in response to static magnetic fields (SMF) used in brain therapy.

Findings

  • The study evaluates medium-intensity SMF effects (305 mT) on a primary culture of cortical astrocytes from neonatal Wistar rats. Stimulation durations were 5, 15, 30, and 40 minutes over seven consecutive days.
  • Results indicated reduced antioxidant capacity for 5 and 40 minutes of stimulation.
  • Increase in lipid peroxidation observed in all stimulated groups, with no significant changes in the enzymatic activities of superoxide dismutase and catalase or total thiol content.
  • Significant alterations in the cell cycle and cell death by necrosis were observed post 15, 30, and 40 minutes of stimulation.
  • Decrease in mitochondrial function, with no marked changes in the mitochondrial network visualized.

Conclusion

The study uncovers potential risks of SMF in therapy, demonstrating compromised oxidative status and cell viability in astrocytes. This urges deeper understanding of SMF impacts at the cellular level, especially considering its widespread use against neurological and psychiatric disorders.

astrocytes static magnetic fields cell viability oxidative stress mitochondrial function
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