Extremely low frequency magnetic field enhances expression of a specific recombinant protein in bacterial host

Authors: Behnaz Rashidieh, Alireza Madjid Ansari, Mahdi Behdani, Behrad Darvishi, Mahdi Habibi-Anbouhi

Year: 2022 May 21

Category: Biochemistry

Journal: Anal Biochem

DOI: 10.1016/j.ab.2022.114745

URL: https://pubmed.ncbi.nlm.nih.gov/35609685/

Abstract

Overview

Protein expression in bacterial host cells, particularly E.coli, is crucial for industrial applications though often hampered by low expression outcomes. The study investigates innovative methods to overcome these limitations.

Findings

  • Extremely Low Frequency Magnetic Fields (ELF-MFs) have a profound impact on biological processes.
  • This research focused on whether ELF-MFs impact overexpression of proteins in E.coli utilizing Cluster of differentiation-22 (CD22) as a model protein.
  • Continuous exposure to ELF-MFs varied in strength and frequency (15-100 mT and 2.5-20 Hz) after applying different IPTG concentrations.
  • A specific frequency range (55mT at 2.5-2.8 Hz) significantly enhanced protein yield across all IPTG levels tested. High or low frequencies outside this range decreased protein expression.

Conclusion

The application of a targeted range of ELF-MFs can be a viable modification for boosting heterologous protein expression in E.coli, presenting a significant advancement for biotechnological applications and safety considerations regarding ELF-MF exposure.

EMF gene expression E.coli protein expression ELF-MFs recombinant protein biotechnology
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