Extremely low frequency magnetic field enhances expression of a specific recombinant protein in bacterial host

Authors: Behnaz Rashidieh, Alireza Madjid Ansari, Mahdi Behdani, Behrad Darvishi, Mahdi Habibi-Anbouhi

Year: 2022 May 21

Category: Biochemistry

Journal: Anal Biochem

DOI: 10.1016/j.ab.2022.114745

URL: https://pubmed.ncbi.nlm.nih.gov/35609685/

Abstract

Overview

Expression of proteins in bacterial host cells such as E.coli is critical, but low expression rates limit industrial application. The study explores the modification of this process using Extremely Low Frequency Magnetic Fields (ELF-MFs).

Findings

  • The study investigated the effects of ELF-MFs on the expression of the model protein Cluster of differentiation-22 (CD22) in E.Coli Rosetta (DE3).
  • Various concentrations of Isopropyl ß-d-1-thiogalactopyranoside (IPTG) were used as an inducer while exposing the bacteria to magnetic fields ranging from 15-100 mT and 2.5-20 Hz.
  • A specific range of 55mT and 2.5-2.8 Hz significantly enhanced protein yield across all IPTG levels tested.
  • Conversely, other EMF intensities resulted in a decline in protein expression especially at higher IPTG levels.

Conclusion

This research concludes that targeted application of ELF-MFs can serve as a novel approach to enhance the heterologous expression of proteins in E.coli, suggesting a potential valuable modification for industrial applications.

ELF-MF protein expression E.coli gene expression recombinant protein magnetic fields
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