Abstract

Overview

The widespread use of cell phones has prompted investigations into their potential adverse effects on human health. This study focuses on the in vivo impacts of radiofrequency-electromagnetic field (RF-EMF) exposure from cell phones on protein expression in rat testicular tissue.

Methodology

A group of 20 Sprague-Dawley rats was subjected to 900 MHz RF-EMF exposure for varying durations—0, 1, 2, or 4 hours per day over a 30-day period. Protein content was analyzed using high-resolution two-dimensional electrophoresis and identified through MALDI-TOF/TOF-MS.

Findings

• Two protein spots, the ATP synthase beta subunit (ASBS) and hypoxia up-regulated protein 1 precursor (HYOU1), were overexpressed significantly after exposure, with induction factors 1.7 times greater post-exposure.
• These proteins are crucial for signaling pathways related to spermatogenesis and protein folding in the endoplasmic reticulum. Their up-regulation suggests potential carcinogenic risks and reproductive damage linked to RF-EMF exposure.

Conclusion

Considering the common practice of carrying cell phones in close proximity to gonads, where exposure can exceed safety guidelines, urgent further studies are recommended to fully understand these effects and mitigate potential health risks.