In vitro study of effects of ELF-EMF on testicular tissues of roe deer (Capreolus capreolus) - FTIR and FT-Raman spectroscopic investigation
Abstract
Overview
Electromagnetic fields (EMF) are widely recognized as an environmental influence on biological tissues. This research primarily focuses on the effects of EMF on the testicular tissues of roe deer evaluated in vitro.
Experiment Details
- Frequencies Tested: 50 Hz and 120 Hz
- Duration of Treatment: 2 hours and 4 hours
- Magnetic Induction Used: 8 mT
Methodology
The study utilized Fourier Transform Infrared Spectroscopy (FTIR) and Fourier Transform Raman Spectroscopy (FT-Raman) to identify the biochemical changes in the tissues by comparing treated samples against control samples.
Findings
The analysis of spectroscopic data revealed significant differences between the treated and control samples. Notably, the intensity and locations of the peaks associated with various functional groups in the tissue samples showed pronounced alterations post EMF exposure.
For instance, with FTIR spectra, 5 out of 15 peaks shifted, while with FT-Raman spectra, 6 out of 10 peaks were affected.
Conclusion
The study concludes that EMF notably impacts the chemical composition of testicular tissues, marking a significant environmental effect on the reproductive biology of male roe deer, potentially with broader implications for other species including humans.