Electromagnetic fields with frequencies of 5, 60 and 120 Hz affect the cell cycle and viability of human fibroblast BJ in vitro

Authors: Koziorowska A, Romerowicz-Misielak M, Filipek A, Koziorowski M

Year: 2017 Jul-Sep

Category: Cell Biology

Journal: J Biol Regul Homeost Agents

URL: https://www.ncbi.nlm.nih.gov/pubmed/28956424

Abstract

Study Overview

The purpose of this research was to investigate the effects of electromagnetic fields (EMFs) on the viability and cell cycle dynamics of human fibroblast BJ cells in vitro.

Experimental Findings

  • Exposure: Cells were exposed to sinusoidal continuous and pulsed EMFs at frequencies of 5 Hz, 60 Hz, and 120 Hz for 2 hours, with a magnetic induction of 2.5 mT.
  • Assessment Methods: Cell viability was evaluated immediately post-exposure and 24 hours later using an MTT assay. Cell cycle changes were analyzed via BrdU incorporation.
  • Results: Pulsed EMFs significantly decreased cell viability across all frequencies, with effects persisting for 24 hours. In contrast, continuous EMF exposure reduced viability only at 120 Hz, with sustained effects after 24 hours.

Conclusion

The study highlights significant variability in cell response based on the nature of the EMF, underscoring the potential health risks associated with different types of electromagnetic exposure.

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