Abstract

Abstract

Overview

More and more studies suggest that prolonged exposure to EMF may cause adverse biological effects and point directly to a significantly negative correlation between EMF and human health, especially men's fertility. In our previous study, we reported that this could be related to the EMF-induced reactive oxygen species formation, followed by DNA damage, cell cycle arrest, and apoptosis induction.

Research Expansion

• In this study, we expanded our research by searching for substances which would prevent EMF-induced damage in spermatogenic cells.
• Aloe arborescens Mill. juice was identified as a potent agent possessing a wide range of protective properties against EMF-induced damage.

Methodology and Findings

The study was conducted using in vitro mouse spermatogenesis pathway cell lines (GC-1 spg and GC-2 spd).

• The administration of aloe extract helps in preventing the formation of free radicals by various biochemical pathways.
• The study provided significant knowledge concerning the mechanism involved in the multi-pathway cytoprotective response of aloe juice against EMF.
• Results suggest that the aloe juice has many positive effects, especially for the cellular antioxidant systems by reducing the intracellular reactive oxygen species pool induced by EMF.

Conclusion

In consequence, aloe juice prevents DNA damage, cell cycle arrest and therefore the viability and metabolic activity of both cell line tested are preserved. This study provides new insight into the underlying mechanisms through which aloe juice prevents spermatogenic cells from cytotoxic and genotoxic events.