Proteomic and Biological Analyses Reveal the Effect on Growth under Flooding Stress of Chickpea Irradiated with Millimeter Waves

Abstract

Overview

Chickpea is a critical food legume grown in arid and semiarid tropics, often hindered by flooding stress. This study explores the dynamic effects of millimeter-wave irradiation on chickpeas under such conditions.

Findings

• Millimeter-wave irradiation was found to improve growth and flooding stress tolerance in chickpeas.
• Proteomic analysis detected variations in proteins associated with photosynthesis, fermentation, and protein degradation.
• Immunoblot analysis indicated changes in RuBisCO activase and large subunits in the leaves under flooding conditions, which were mitigated by irradiation.
• Enhanced activity and accumulation of alcohol dehydrogenase were observed in the roots, mirroring responses in above-ground plant parts.
• Significant reduction in cell death was observed in irrigated chickpeas as compared to unirradiated ones under flooding conditions.

Conclusion

These findings underscore the potential benefits of millimeter-wave irradiation in improving chickpea resilience to environmental stressors, highlighting a pivotal role in regulating key biological processes.