Abstract

Overview

Our research focuses on the effects of low-frequency magnetic fields (LF-MFs) on cell viability, specifically through mechanisms involving calcineurin-mediated signaling pathways influenced by reactive oxygen species (ROS-like molecules).

Experiments

• Two leukocytic cell lines, Mono Mac 6 and U937, were selected for experimentation.
• Cells were exposed to various LF-MFs in combination with puromycin (PMC).
• Western Blot analysis was used to study the expression of key regulatory proteins of calcium metabolism.
• Cell viability and ROS levels were assessed using cytochrome C reduction assay and flow cytometry.

Findings

The presence of magnetic fields and PMC notably influenced cell viability in a MF-dependent manner. This impact was seen as directly correlated with changes in protein expression and reactive oxygen species levels. It was experimentally confirmed that the applied stress stimuli increased cell susceptibility to death.

Conclusion

The observed bioeffects are distinct and specific to differing leukocyte populations, highlighting the crucial influence of LF-MFs on cellular functions and health risks.