Exposure of human lymphocytes to sweeping-frequency extremely low frequency magnetic field

Authors: Zastko L, Makinistian L, Petrovicová P, Tvarožná A, Belyaev I

Year: 2025 Aug 7

Category: Radiobiology

Journal: International Journal of Radiation Biology

DOI: 10.1080/09553002.2025.2542322

URL: https://pubmed.ncbi.nlm.nih.gov/40772784/

Abstract

Overview

This study examines how exposure to sweeping-frequency extremely low frequency magnetic fields (ELF MF) impacts human lymphocytes.

Study Design & Methods

  • Human umbilical cord blood lymphocytes (UCBL) were isolated and exposed for 48 hours to a sinusoidal ELF MF sweeping from 3–26 Hz.
  • Six different amplitudes were tested, ranging from 6 μT to 24 μT, using an oblong coil.
  • DNA double-strand breaks (DSB) were evaluated by assessing γH2AX, 53BP1, and co-localized γH2AX/53BP1 DNA repair foci.
  • Cell viability and apoptosis stages were measured, identifying viable cells, early apoptotic (EA), and late apoptotic/necrotic (LAN) cells.

Findings

  • No statistically significant effects on DNA repair foci or apoptosis induction were observed after 48 hours of ELF MF exposure.
  • A notable finding: at 8 μT amplitude, there was more than a two-fold decrease (p = .064) in γH2AX foci compared to controls, suggesting reduced DNA double-strand breaks.
  • Additionally, for the same field intensity, data pointed to a decrease in viable cell count.

Conclusion

These findings suggest potential preventive or blocking effects of sweeping-frequency ELF MF on DNA double-strand break (DSB) formation in lymphocytes. This indicates a possibility of using such magnetic fields as a protective measure against genotoxic agents and highlights the importance of thoroughly testing frequency and intensity variations for optimal biological effects.

Note: Any exposure to electromagnetic fields can influence biological systems, and this study signals the relevance of further research to fully understand both potential health risks and protective effects of ELF MF exposures.

ELF magnetic fields human lymphocytes DNA damage apoptosis electromagnetic exposure ion-cyclotron frequencies health effects
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