Biochemical, immunohistochemical and morphometrical investigation of the effect of thymoquinone on the rat testis following exposure to a 900-MHz electromagnetic field

Authors: Yahyazadeh A, Altunkaynak BZ, Kaplan S

Year: 2019 Nov 26

Category: Biochemistry, Immunohistochemistry

Journal: Acta Histochem

Institution: Acta Histochem

DOI: 10.1016/j.acthis.2019.151467

URL: https://www.ncbi.nlm.nih.gov/pubmed/31784235

Abstract

Abstract Summary

Overview

Concerns about public health have been heightened by the long-term use of cell phones, which emit electromagnetic fields (EMFs). This study focuses on the potential impacts of 900 MHz EMF exposure on rat testis, with specific attention to mitigation strategies using thymoquinone (TQ).

Methodology

Experiment Design: Twenty-four male adult Wistar albino rats were organized into four groups:

  • Control
  • EMF Exposed
  • TQ Treated
  • EMF + TQ Treated

Duration: The exposure lasted for 60 minutes per day over a period of 28 days. TQ was administered at 10 mg/kg/day.

Findings

Testicular Analysis: Testes from the EMF-exposed rats showed significant reductions in primary spermatocytes, spermatids, and Leydig cells compared to the control group. However, the group treated with both EMF and TQ showed a relative improvement in these numbers.

Biochemical Results: Increased activity of superoxide dismutase (SOD) and lower testosterone levels and testes weight were noted in the EMF group compared to the control group. These indicators collectively suggest oxidative stress due to EMF exposure.

Conclusion

The study confirms that 900 MHz EMF exposure results in harmful effects on rat testicular tissue, and emphasizes that using TQ might partly reduce these damages. It underscores the health risks associated with EMF radiation from common devices like cell phones.

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