Mitochondrial DNA damage and oxidative damage in HL-60 cells exposed to 900MHz RF

Abstract

Overview

HL-60 cells derived from human promyelocytic leukemia were investigated for potential dangers posed by exposure to 900MHz radiofrequency fields.

Methodology and Exposure

The cells were subjected to 120μW/cm2 power intensity of continuous wave radiofrequency for 4 hours per day across a span of five consecutive days.

Key Findings

• Significant increase in reactive oxygen species (ROS).
• Several decreases noticed in mitochondrial functionality including transcription factor A, mitochondrial DNA (mtDNA) polymerase gamma, mtDNA transcripts, and mtDNA copy number.
• Notable increase in 8-hydroxy-2'-dexoyguanosine (8-OHdG) levels, a key marker for oxidative damage.
• Reduction in the synthesis of adenosine triphosphate (ATP), crucial for cellular functions.
• Exposure results were somewhat similar to those observed in cells treated with gamma radiation (GR), also known to induce ROS.

Protective Measures and Conclusion

Prior-treatment with melatonin, an effective free radical scavenger, demonstrated reversed effects in RF-exposed cells, highlighting a potential mitigative approach against RF-induced cellular damage.