Genomic DNA damage induced by co-exposure to DNA damaging agents and pulsed magnetic field

Abstract

Overview

The study examines the effects of extremely low-frequency magnetic fields (MF) on DNA damage, with a special focus on understanding the mechanism by which MF interacts with living organisms. Previous research has hinted at MF's potential to enhance the production of Reactive Oxygen Species (ROS), though these interactions remain largely unconfirmed.

Materials and Methods

• Biological Material: Genomic DNA extracted from S. cerevisiae cultures.
• Exposure: Subjected to pulsed magnetic fields (1.5 mT peak, 25 Hz) and DNA damaging agents (MMS 0-1%), for various durations (15-60 minutes) and similarly, to MF and bleomycin (0-0.6 IU/ml) spanning 24-72 hours.
• Evaluation Technique: DNA damage assessed using electrophoresis and image analysis.

Findings

Exposure to pulsed MFs significantly increased the level of DNA damage in conjunction with the use of DNA damaging agents like MMS and bleomycin across all experimental conditions.

Conclusion

Pulsed MFs potentially modulate the cytotoxic actions of chemical agents like MMS and bleomycin. This modulation seems to be a complex interaction influenced by the type of DNA-damaging agent, dosage, and exposure duration to pulsed MFs.