Effect of the Electromagnetic Field (EMF) Radiation on Transcriptomic Profile of Pig Myometrium during the Peri-Implantation Period-An In Vitro Study

Authors: Ewa Monika Drzewiecka, Wiktoria Kozlowska, Lukasz Paukszto, Agata Zmijewska, Pawel Jozef Wydorski, Jan Pawel Jastrzebski, Anita Franczak

Year: 2021 Jul 7

Category: Molecular Biology

Journal: Int J Mol Sci

DOI: 10.3390/ijms22147322

URL: https://pubmed.ncbi.nlm.nih.gov/34298942/

Abstract

Overview

The study investigates the impact of electromagnetic fields (EMF) on the physiological processes in mammals, specifically focusing on the transcriptomic alterations in pig myometrium during the peri-implantation period.

Methodology

Using next-generation sequencing, the research explores the transcriptomic changes following a short-term (2 hours) exposure to EMF (50 Hz, 8 mT).

Findings

  • Identification of 215 transcript active regions (TARs), with 90 being protein-coding genes that showed differential expression.
  • Notable changes were observed in genes associated with defense and immune responses, and secretion and export.
  • The study further evaluated pathway enrichments like the KEGG TNF signaling pathway, and regulation of IFNA signaling and interferon-alpha/beta signaling in the REACTOME pathways.
  • Detection of 12 differentially expressed long non-coding RNAs (DE-lnc-RNAs) and 182 predicted single nucleotide variants (SNVs) substitutions in RNA editing sites.

Conclusion

The exposure to EMF significantly alters gene expression in myometrium during the peri-implantation period, particularly affecting defense and immune response genes. It also provides insights into the molecular mechanisms of EMF influence through lnc-RNAs and SNVs.

EMF transcriptomics myometrium gene expression RNA sequencing
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