2.4 GHz Electromagnetic Field Influences the Response of the Circadian Oscillator in the Colorectal Cancer Cell Line DLD1 to miR-34a-Mediated Regulation

Authors: Olejárová S, Moravčík R, Herichová I

Year: 2022 Oct 30

Category: Molecular Biology

Journal: Int J Mol Sci

DOI: 10.3390/ijms232113210

URL: https://www.mdpi.com/1422-0067/23/21/13210

Abstract

Overview

The influence of Radiofrequency electromagnetic fields (RF-EMF) on biological processes, including circadian rhythms, is significant. This study focuses on miR-34a, a small non-coding RNA, which is modulated by RF-EMF and can regulate clock gene expression in colorectal cancer cells.

Findings

  • miR-34a was observed to modulate key clock genes such as per2, bmal1, sirtuin1, and survivin in DLD1 cells, affecting their proliferation and migration negatively.
  • Exposure to 2.4 GHz RF-EMF altered the impact of miR-34a, showing increased cry1 mRNA expression, weakened inhibition of per2, and elimination of survivin's inhibition.
  • The study utilized real-time PCR, scratch assay test, and MTS test to evaluate these effects.

Conclusion

This research suggests that RF-EMF exposure could negate or diminish the tumor-suppressive effects of miR-34a in colorectal cancer cells, highlighting the antagonistic interplay between RF-EMF and miRNA-mediated regulation of cancer pathways. These findings align closely with the WHO classification of RF-EMF as a possible carcinogen and indicate the potential of RF-EMF to alter tumor behavior in critical ways.

RF-EMF circadian rhythms cancer cell lines miRNA gene expression
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