Abstract

Overview

An electromagnetic field (EMF) may have effects on female reproduction. This study aims to investigate the impact of EMF radiation on androgen synthesis and release in pig endometria during fetal peri-implantation.

Methodology

• The experiment utilized endometrial slices from pigs during days 15-16 of gestation, treated in vitro. EMF exposures were at frequencies of 50 and 120 Hz over periods of 2 and 4 hours, both with and without the presence of progesterone.
• Techniques: Real-Time PCR and Western Blot to measure mRNA and protein abundance.

Findings

• Progesterone partially influence EMF impact, showing a protective role against T (testosterone) release suppression after 2 and 4 hours of EMF exposure but enhancing A4 (androstenedione) release after 4 hours at 120 Hz.
• Without progesterone, the release of A4 decreased after shorter exposure times and across all tested frequencies after 4 hours.

Conclusion

EMF radiation selectively suppresses androgen release and alters androgen levels depending on exposure duration and frequency, as well as the hormonal environment. This highlights significant potential risks of EMF on reproductive health which needs further investigation.