Effect of the Electromagnetic Field (EMF) Radiation on Transcriptomic Profile of Pig Myometrium during the Peri-Implantation Period-An In Vitro Study

Authors: Ewa Monika Drzewiecka, Wiktoria Kozlowska, Lukasz Paukszto, Agata Zmijewska, Pawel Jozef Wydorski, Jan Pawel Jastrzebski, Anita Franczak

Year: 2021 Jul 7

Category: Molecular Biology

Journal: Int J Mol Sci

Institution: Int J Mol Sci

DOI: 10.3390/ijms22147322

URL: https://pubmed.ncbi.nlm.nih.gov/34298942/

Abstract

Overview

The electromagnetic field (EMF) is known to influence physiological processes in mammals. Despite observable effects, the molecular mechanisms underpinning these changes are not fully understood.

Findings

This study explored the impact of a short-term EMF exposure (2 hours at 50 Hz, 8 mT) on the global transcriptomic alterations in the myometrium of pigs during the peri-implantation period. Advanced next-generation sequencing techniques were employed to analyze these effects.

EMF exposure resulted in changes in 215 transcript active regions (TARs).
Among these, 90 were identified as differentially expressed genes (DEGs) involved predominantly in defense and immune responses as well as secretion and export processes.
The study highlighted significant enrichment in the KEGG TNF signaling pathway and adjustments in IFNA and interferon-alpha/beta signaling according to REACTOME pathways.
12 differentially expressed long non-coding RNAs (DE-lnc-RNAs) and 182 predicted single nucleotide variants (SNVs) substitutions within RNA editing sites were identified, indicating potential novel regulatory mechanisms.

Conclusion

EMF exposure during the peri-implantation period significantly alters gene expression related to important physiological functions, implicating potential health risks and biological effects. The findings provide new insights into the molecular actions of EMF and its regulatory impact through lnc-RNAs and SNVs.

← Back to Stats