Abstract

Overview

Our research focuses on the effects of microwave radiation on the male reproductive system, particularly the role of blueberry anthocyanins in protecting against oxidative stress injuries.

Findings

• The study confirms the ability of blueberry anthocyanins to mitigate damage in the testicular tissue structure, improve sperm motility, sex hormone levels, and antioxidative markers such as GSH/GSSG and SOD while decreasing MDA in Wistar rats.
• Key molecular changes include increased SIRT1 expression and reduced FoxO1 expression.
• Energetic metabolism and cell survival were enhanced as indicated by increased levels of LDH, SDH, ATP synthase, and decreased Caspase-3 expression.
• Among the doses tested, 400 mg/kg/day was identified as the most effective for providing protective benefits.

Conclusion

The results suggest that blueberry anthocyanins could serve as a protective agent against oxidative stress injury in rat testicular tissues induced by 2.856 GHz microwave radiation, by modulating the SIRT1/FoxO1 pathway and enhancing cellular energy and survival mechanisms.