Retrospective estimation of ELF & MF exposure in in vitro studies reveal considerable potential for uncertainty

Abstract

Overview

Experiments involving cell cultures exposed to extremely low frequency (ELF, 3-300 Hz) magnetic fields often deal with multiple uncertainties related to specific exposure conditions.

Findings

• Quantitative analysis of uncertainties was conducted based on descriptions of exposure conditions in various bioelectromagnetic experimental reports.
• Identified uncertainties largely originate from insufficient, ambiguous, or erroneous descriptions and implementations of experimental methods and systems.
• These issues often render successful reproduction of original experimental conditions challenging or unachievable.

Conclusion

There is a significant need to improve the reporting and execution of experimental exposure conditions in bioelectromagnetics research. Such improvements are crucial to ensure reproducibility and understand the biological effects of ELF magnetic fields. The findings underscore the vital role of careful experimental design and validation to reduce variability and uncertainty in bioelectromagnetic studies.