The endocannabinoid system is involved in the anxiety-like behavior induced by dual-frequency 2.65/0.8 GHz electromagnetic radiation in mice

Abstract

Overview

With the increasing popularity of wireless communication devices, the potential mental health risks associated with environmental exposure to electromagnetic radiation (EMR), specifically complex frequency EMR, have emerged as a significant public health concern.

Research Focus

This study shifts focus from the commonly studied single-frequency EMR to dual-frequency and multi-frequency EMRs to better mimic real-world exposure. We aim to delineate the emotional impacts of these frequencies and explore underlying molecular mechanisms and potential targets for intervention.

Findings

• Exposure to single-frequency EMR at either 2.65 GHz or 0.8 GHz did not cause anxiety-like behaviors in mice.
• In contrast, dual-frequency EMR at 2.65/0.8 GHz led to significant anxiety-like behaviors.
• Further tests indicated increased levels of corticosterone and corticotrophin releasing hormone.
• Transcriptome sequencing and subsequent analyses showed a decrease in cannabinoid receptor 1 (CB1R) expression and content, alongside a reduction in the endocannabinoid 2-arachidonoylglycerol in the cerebral cortex.
• Administration of cannabinoid receptor agonist and antagonist, respectively, alleviated or reversed the anxiety-like behaviors.

Conclusion

The study underscores dual-frequency EMR's ability to induce anxiety-like behavior in mice, implicating the hypothalamic-pituitary-adrenal axis and the endocannabinoid system. This suggests potential therapeutic approaches involving cannabinoid receptor modulation to counteract EMR-induced anxiety.