Protective Effects of Zinc on 2.45 GHz Electromagnetic Radiation-Induced Oxidative Stress and Apoptosis in HEK293 Cells

Authors: Özsobacı NP, Ergün DD, Tunçdemir M, Özçelik D

Year: 2019 Jul 17

Category: Cell Biology

Journal: Biol Trace Elem Res

Institution: Biol Trace Elem Res

DOI: 10.1007/s12011-019-01811-6

URL: https://www.ncbi.nlm.nih.gov/pubmed/31317470

Abstract

Overview

Electromagnetic radiation (EMR) has been demonstrated by various epidemiological studies to pose significant health risks.

Findings

This research investigated the impact of 2.45 GHz EMR on human kidney embryonic cells (HEK293). The study primarily focused on oxidative stress and apoptotic responses in cells subjected to EMR, assessing the potential protective role of zinc (Zn). The experimental setup included different groups: control, EMR-only, and two EMR groups treated with varying concentrations of zinc (50 μM and 100 μM).

  • Oxidative stress was quantified spectrophotometrically, with findings showing increased malondialdehyde (MDA) levels and decreased superoxide dismutase (SOD) activity in the EMR group compared to controls.
  • Apoptotic indices, including caspase-3 activity and TUNEL assay results, indicated increased apoptosis in the EMR group.
  • Zinc treatment reduced MDA levels and caspase-3 activity, increased SOD activity and enhanced bcl-2 immunopositivity, suggesting a protective effect against EMR-induced cellular damage.

Conclusion

The study concludes that zinc supplementation can mitigate some of the deleterious effects of EMR on HEK293 cells, highlighting its potential as a protective agent against EMR-induced oxidative stress and apoptosis.

electromagnetic radiation oxidative stress apoptosis zinc HEK293 cells protective effects
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