Effects of moderate static magnetic fields on voltage-gated potassium ion channels in sympathetic neuron-like PC12 cells

Authors: Kaneda E, Kawai T, Okamura Y, Miyagawa S

Year: 2025 Mar

Category: Neuroscience

Journal: Physiological Reports

DOI: 10.14814/phy2.70236

URL: https://physoc.onlinelibrary.wiley.com/doi/10.14814/phy2.70236

Abstract

Overview

Exposure to moderate static magnetic fields (SMF) has been shown to alter neuronal excitability, yet the specific effects on sympathetic neurons have been largely unstudied. This investigation evaluates how moderate SMF affects voltage-gated potassium (Kv) channels in the plasma membrane of sympathetic neuron-like PC12 cells.

Findings

  • Kv channel current density was significantly reduced in PC12 cells subjected to 18 hours of magnet exposure.
  • This reduction persisted after the magnetic field was removed, indicating a lasting effect on channel function.
  • Outward current in the presence of tetraethylammonium (TEA) showed no difference between groups, supporting the involvement of TEA-sensitive Kv channels in the observed effect.
  • RNA sequencing identified 37 genes sensitive to moderate SMF exposure.
  • KEGG pathway analysis and mRNA expression indicated upregulation of pathways that inhibit neuronal excitability, such as GABAB receptor activation and Kir3 channel opening.

Conclusion

Moderate SMF exposure is strongly associated with reduced current density in PC12 cells, specifically affecting Kv channels. This study highlights a fundamental link between long-term SMF exposure and altered excitability in sympathetic neurons, suggesting a tangible impact of electromagnetic field exposure on neuronal properties.

static magnetic fields potassium channels sympathetic neurons PC12 cells neuronal excitability electrophysiology gene expression
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