Abstract

Overview

The study investigates the interactions between cells and extremely low frequency magnetic fields (ELF-MFs), focusing on oxidative stress induction in SH-SY5Y human neuroblastoma cells.

Methods

A computational multiscale approach was employed to estimate induced electric fields and current density, considering cell morphological models and different cellular arrangements. Additionally, the study integrates the use of Diphenyleneiodonium (DPI), an inhibitor of the plasma membrane enzyme NADPH oxidase (Nox), which was administered before ELF exposure for experimental validation.

Findings

• An increase in current density at the plasma membrane/extra-cellular medium interface indicates the plasma membrane as the primary interaction site.
• DPI treatment significantly mitigates the generation of reactive oxygen species induced by ELF exposure, confirming the pivotal role of membrane Nox in redox imbalances under ELF influence.

Conclusion

This study highlights the significant role of microscopic current densities induced at the plasma membrane in the redox imbalance associated with ELF exposure. The employ of multiscale computational dosimetry, combined with an in vitro validation approach, offers a new paradigm for understanding the complex interactions between ELF-MFs and biological systems.