Exposure to 60 Hz magnetic field can affect membrane proteins and membrane potential in human cancer cells

Abstract

Overview

This study explores the impact of extremely low frequency magnetic fields (ELF-MF) on cell membranes, specifically focusing on the effects of a 60 Hz, 50 mT magnetic field on membrane potential and proteins in cultured human cancer cells.

Findings

• Increased Membrane Potential: Exposure to ELF-MF increased membrane potentials in three human cancer cell lines: A549, MES-SA, and MES-SA/Dx5.
• Interaction with Anticancer Drugs: Changes in membrane potentials were observed in A549 and MES-SA cells when exposed to both MF and an anticancer drug, but not in the drug-resistant MES-SA/Dx5 cells.
• Protein Structure Changes: The structure of hydrophobic sites on membrane proteins altered, increasing their likelihood to bind with DiBAC4(3) dye following a 10-minute MF exposure. However, a decrease in fluorescence was noted after 240 minutes of exposure, indicating varied effects based on exposure duration.

Conclusion

The study supports the view that magnetic fields, specifically at 60 Hz and 50 mT, are influential to the biological functions of human cancer cells, indicating potential health risks associated with prolonged exposure to ELF-MF.