Abstract

Overview

This research investigates the biological impacts of extremely low-frequency electromagnetic fields (ELF-EMFs) on human health, specifically focusing on changes in antioxidant gene expression within human MCF-7 cells.

Methodology

The study utilizes EMF exposure patterns varying in duration and field intensity simulated in a controlled environment. The mRNA levels of seven key antioxidant genes were measured post-exposure using real-time polymerase chain reactions.

Findings

• Three distinct exposure conditions were tested each aiming for a total exposure time of 30 minutes: 5 minutes on/off, 15 minutes on/off, and 30 minutes on continuously.
• Two field intensities, 0.25 mT and 0.50 mT, were analyzed.
• Significant gene expression changes were recorded at 0.50 mT during the 15 minutes on/off exposure condition, showcasing a potential increase in both down and up regulation of gene activity compared to other tested conditions.
• No cytotoxic effects were observed in any testing condition, indicating the cell viability was maintained.

Conclusion

The increased regulation of genes at higher field intensities suggests a noteworthy effect of ELF-EMFs on antioxidant gene behavior, highlighting potential concerns regarding continued ELF-EMF exposure in human environments.