ELF EMFs Affect Myogenic Processes in C2C12 Myoblasts: Role of Gap-Junction-Mediated Intercellular Communication

Abstract

Overview

Extremely low-frequency electromagnetic fields (ELF-EMFs) are known to interact with biological systems. While they are used therapeutically in physical therapy and rehabilitation, their role as potential environmental pollutants poses a public health concern.

Findings

• The study utilized C2C12 myoblasts, exposing them to ELF-EMFs generated by a solenoid.
• Assessments included cell viability and proliferation, studying changes in growth rates and differentiation through colorimetric and vital dye assays, as well as cytomorphological and molecular analyses.
• Significant findings included the maintenance of cell viability and the lack of effect on the proliferation rate under ELF-EMF exposure.
• Increased expression of MyoD, myogenin, and connexin 43 was noted. Enhanced gap-junction function was suggested to promote myoblast fusion into myotubes and accelerate the myogenic process.

Conclusion

This investigation offers initial insights into the mechanisms by which ELF-EMFs aid therapeutic outcomes and potentially alleviate muscle dysfunction conditions. Though beneficial in therapy, the environmental influence and health implications of ELF-EMFs as potential pollutants require further detailed examination.