Abstract

Overview

The burgeoning use of communication devices raises concerns about the effects of radiofrequency electromagnetic radiation (RF-EMR), particularly at 1,800 MHz on brain development.

Background

Radiofrequency electromagnetic radiation (RF-EMR), with a global surge in mobile phone usage, is feared to impact the brain, a vulnerable organ, at 1,800 MHz exposure levels.

Objectives

• To examine the impact of 48-hour exposure to 1,800 MHz RF-EMR on neurite outgrowth in neuronal cells.
• To explore the role of the Rap1 signaling pathway in this context.

Materials and Methods

Using C57BL/6 mouse primary hippocampal neurons and Neuro2a cells, the research involves RF-EMR exposure at a SAR of 4 W/kg over 48 hours. Various assays and imaging techniques were employed to measure effects on neurite outgrowth and examine Rap1 signaling pathway involvement.

Findings

• Exposure did not affect cell viability but significantly impaired neurite outgrowth and activity of Rap1.
• Overexpression of constitutively active Rap1 counters the RF-EMR-induced impairments.

Conclusion

Changes in Rap1 activity and associated signaling pathways suggest a negative impact of RF-EMR exposure on neurite outgrowth, highlighting the need for detailed studies on its effects on children’s neuronal development.