Molecular Mechanism of Malignant Transformation of Balb/c-3T3 Cells Induced by Long-Term Exposure to 1800 MHz Radiofrequency Electromagnetic Radiation (RF-EMR)
Abstract
Overview
This study investigates the potential hazards of long-term exposure to 1800 MHz Radiofrequency Electromagnetic Radiation (RF-EMR) and its ability to induce malignant transformation in Balb/c-3T3 cells.
Methods
- Exposure of Balb/c-3T3 cells to 1800 MHz RF-EMR for 40 and 60 days, 4 hours daily.
- Control group sham-exposed to assess comparability.
- Assessment techniques included cell transformation assays, transplantation in mice, clone formation, and transwell assays.
- Use of mRNA microarray assays to identify critical genes and pathways.
Findings
The exposed cells showed significant increases in proliferation and migration, with clear signs of malignant transformation. Crucially, exposed cells formed visible tumors in immune-deficient mice and exhibited major alterations in lipid metabolism, specifically the mevalonate pathway.
Conclusion
Extended exposure to RF-EMR significantly contributes to the malignant transformation of cells, with potentially profound implications for health safety regulations regarding electromagnetic field exposure. Further exploration of the underlying molecular mechanisms is recommended.