Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on Genotoxicity & Heat Shock Protein Expression of Cells Derived from Human Eye

Authors: Koyama S, Narita E, Shimizu Y, Suzuki Y, Shiina T, Taki M, Shinohara N, Miyakoshi J.

Year: 2016 Aug 8

Category: Environmental Health, Public Health

Journal: Int J Environ Res Public Health

DOI: 10.3390/ijerph13080802

URL: https://www.ncbi.nlm.nih.gov/pubmed/27509516

Abstract

Overview

This study explores the impact of 60 gigahertz (GHz) millimeter-wavelength radiation on cells derived from the human eye. The goal was to assess potential genotoxic effects and changes in heat shock protein (Hsp) expression following long-term exposure.

Methodology

  • Cell Types: Human corneal epithelial (HCE-T) and human lens epithelial (SRA01/04) cells.
  • Exposure: Cells were exposed to 60 GHz radiation for 24 hours at an intensity of 1 mW/cm².
  • Control Groups: Sham-exposed controls and incubator controls.

Findings

No significant increases were observed in the micronucleus (MN) frequency in exposed cells compared to controls. Positive control was provided by cells treated with bleomycin, validating the test's sensitivity. Additionally, assessments of DNA strand breaks using the comet assay and examination of heat shock protein expression showed no significant effects from the radiation exposure.

Conclusion

The findings indicate that exposure to 60 GHz millimeter-wavelength radiation does not increase genotoxicity or alter heat shock protein expression in human eye cells under the conditions tested. This suggests a lack of significant health risks associated with such radiation exposure in human eye cells.

60 GHz radiation human eye cells genotoxicity heat shock protein millimeter-wavelength cell biology
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