Abstract

Overview

In this study, we investigate the biological impact of long-term exposure (5 months) to static and extremely low frequency magnetic fields on Dubia roach nymphs, focusing on changes in specific biomarkers.

Findings

• Analyzed the effects of a static magnetic field (SMF) of 110 mT and an extremely low frequency magnetic field (ELF MF) of 10 mT at 50 Hz.
• Focus on acetylcholinesterase (AChE) activity and the level of heat shock protein 70 (HSP70), identified as stress biomarkers in insects.
• Methods included spectrophotometric measurements of AChE and quantification of HSP70 through ELISA and Western blotting.
• Significant changes in relative growth rate (RGR) observed, hinting at the developmental impacts of magnetic field exposure.
• Decreased AChE activity noted in exposed nymphs, indicative of neurological stress or damage.
• Increase in HSP70 levels was specifically notable only with SMF exposure, suggesting a field strength-dependent response in stress protein expression.
• Differential HSP70 isoform responses to SMF exposure, pointing to a potential adaptive mechanism or damage response specific to magnetic field characteristics.

Conclusion

The study highlights the sensitive nature of Blaptica dubia nymphs to chronic electromagnetic field exposure, emphasizing significant biological alterations. These findings contribute to understanding the broader implications of electromagnetic fields on living organisms, reinforcing the need for cautious evaluation of EMF effects on health.