Protective properties of myrtle extract against oxidative effects of ELF EMF on rat plasma and hemoglobin

Abstract

Overview

This study assesses the protective capabilities of Myrtus communis extract against the oxidative effects induced by Extremely Low Frequency Magnetic Fields (ELFMF) on rat plasma and hemoglobin.

Materials and Methods

• Subjects: Adult male rats split into three groups: control, ELFMF exposure, and ELFMF exposure with Myrtus communis extract administration.
• Procedure: Application of magnetic field (0.7 mT, 50 Hz) and injection of Myrtus communis extract at 0.5 mg/kg intraperitoneally prior to exposure.
• Assessment Techniques: Analysis of hemoglobin and plasma through oxidative damage markers like methemoglobin (metHb) levels and protein carbonyl (PCO) levels.
• Statistical Analysis: One-way ANOVA and artificial neural network (ANN) for predictive parameter assessment.

Findings

• Oxidative Impact: Notable increase in metHb and PCO levels upon exposure to ELFMF. Reduction in the antioxidant power of plasma.
• Protective Effect of Extract: Significant reduction in oxidative markers and improved plasma antioxidant levels after administration of Myrtus communis extract.
• ANN Predictions: Effective in estimating the oxyhemoglobin concentration from hemoglobin interaction parameters under stress conditions.

Conclusion

The Myrtus communis extract substantially enhances the erythrocytes' and plasma's capacity to manage oxidative conditions induced by ELFMF exposure. Moreover, ANN models effectively predict key parameters influencing hemoglobin structure during oxidative stress.