Effects Induced by a Weak Static Magnetic Field of Different Intensities on HT-1080 Fibrosarcoma Cells

Abstract

Overview

In this study, we investigated the effects of weak static magnetic fields (SMFs) on HT-1080 human fibrosarcoma cells, focusing on various cellular processes such as growth rates, oxidative stress, and intracellular signaling molecules.

Methodology

The experiment exposed HT-1080 cells to SMFs ranging from 0.5 to 600 µT over four days, with growth and biochemical markers compared against a control exposure of 45 µT.

Findings

• Growth Rate: Varied with SMF intensity; higher fields generally increased growth rates apart from specific dips observed.
• Oxidative Stress: Increased systematically with the rise in SMF intensity, indicative of heightened reactive oxygen species (ROS) activity.
• Mitochondrial Changes: Notable increases in mitochondrial calcium and membrane potential were observed, alongside a decrease in intracellular pH.
• Reactive Molecules: Concentrations of hydrogen peroxide (H2O2), nitric oxide (NO), and superoxide varied, showing complex interaction patterns with SMF intensities.

Conclusion

The study revealed that SMFs can impact fibrosarcoma cell growth and biochemical state by altering ROS levels and other mitochondrial signals. These effects might contribute to both potentially beneficial and adverse impacts on biological systems, highlighting the importance of understanding EMF's broad implication on health, particularly concerning diseases like cancer and aging.